Monitoring DNA double strand breaks repair protiens before and after gamma-irradiation

In this study, different fluorescence techniques (FCS, RICS and FRAP) are used to study kinetics and dynamics of DNA double stand breaks repair proteins before and after gamma-irradiation of mammalian cells. Diffusion and binding constants were obtained by fitting with different physical models. This work presents similarities and differences in double strand break repair response between gamma-irradiation versus laser damage.