Synthesis of fluorescent gold nanoparticle and their application in the photothermal treatment of HCC 1395 cell lines

Monodispersed, fluorescent gold nanoparticles with~gallic~acid based ligand shells are synthesized at~various~concentrations with an average particle size of~1-2nm.~Monodispersity and particle~size are confirmed via dynamic light scattering. The Surface Plasmon Resonance (SPR) peak is determined via UV-Vis. Afterwards~HCC 1395~cells, derived from breast cancer cells,~are infused with the gold nanoparticles. Results are presented on how~infusion with~gold nanoparticles and~photothermal~treatment affect the cell viability. The~photothermal~treatment of the solution is performed at 532nm with a~MGL~III 532-300mW~laser near the SPR peak over different time intervals.~The photothermal~treatment of the solution results in a locally raised temperature and can possibly affect cell viability. After photothermal treatment and incubation for 24hours,~the cells are stained and cell viability is analyzed.