Techniques for super-resolution microscopy using NV-diamond

ORAL

Abstract

We discuss the development and application of techniques for super-resolution microscopy using NV centers in diamond: stimulated emission depletion (STED), metastable ground state depletion (GSD), and stochastic optical reconstruction microscopy (STORM). NV centers do not bleach under optical excitation, are not biotoxic, and have long-lived electronic spin coherence and spin-state-dependent fluorescence. Thus NV-diamond has great potential as a fluorescent biomarker and as a magnetic biosensor.

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Authors

  • Alexei Trifonov

    Harvard, Harvard University

  • David Glenn

    Harvard-Smithsonian, Harvard-Smithsonian Center for Astrophysics, Harvard University

  • Nir Bar-Gill

    Harvard-Smithsonian, Harvard-Smithsonian Center for Astrophysics, Harvard University

  • David Le Sage

    Harvard-Smithsonian, Harvard University

  • Ronald Walsworth

    Harvard-Smithsonian Center for Astrophysics; Department of Physics, Harvard University, Harvard-Smithsonian Center for Astrophysics, Harvard-Smithsonian, Harvard University