Proper Orthogonal Decomposition of flagellar motion in sperm to reconstruct and compare typical beat patterns

ORAL

Abstract

Internally-driven flagella in sperm beat with a complex waveform. We extract flagellar beating patterns from high-speed, high-resolution videos of flagellar beating in tethered mouse sperm. Proper Orthogonal Decomposition is used to resolve beat patterns into major shape modes. Typical beat patterns are constructed by averaging the time-dependent amplitudes of the major shape modes. The Slender Body Theory is applied to obtain typical surface traction and hydrodynamic power distributions across sperm flagella. It is observed that sperm from wild-type mice and CRISP2-deficient mutants have similar major shape modes but differ in their amplitudes. The power distribution curves are thus different for the two species indicating a change in the location of the maximal power dissipation due to the mutation.

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Presenters

  • Ashwin Nandagiri

    • IITB-Monash Research Academy, Mumbai, 400076, India

Authors

  • Ashwin Nandagiri

    • IITB-Monash Research Academy, Mumbai, 400076, India

  • Avinash Gaikwad

    • School of Biological Sciences, Monash University, Clayton,VIC,3800, Australia

  • Moira O'Bryan

    • School of Biological Sciences, Monash University, Clayton,VIC,3800, Australia

  • Julio Soria

    • Department of Mechanical & Aerospace Engineering, Monash University, Clayton,VIC,3800, Australia
    • Monash Univ, King Abdulaziz University
    • Monash University
    • Laboratory for Turbulence Research in Aerospace and Combustion, Monash University
    • Laboratory for Turbulence Research in Aerospace and Combustion, Department of Mechanical and Aerospace Engineering, Monash University, Melbourne 3800, Australia

  • Ranganathan Prabhakar

    • Department of Mechanical & Aerospace Engineering, Monash University, Clayton,VIC,3800, Australia
    • Department of Mechanical and Aerospace Engineering, Monash University, Clayton, VIC 3800, Australia

  • Sameer Jadhav

    • Department of Chemical Engineering, Indian Institute of Technology Bombay, Mumbai, 400076, India
    • Department of Chemical Engineering, IIT Bombay, Mumbai, India