Study of rapid annealing of DNA origami structures.

While DNA origami technology has been around for more than a decade, questions remain about the reaction parameters necessary for promoting efficient folding reactions and good product quality. In particular, conventional DNA origami fabrication relies on slow thermal ramping, which limits throughput and requires specialized equipment. Here we evaluate an isothermal approach for rapid self-assembly of DNA origami nanostructures in which folding occurs at a fixed temperature rather than during gradual cooling. This methodology allows for much more rapid fabrication of self-assembled structures but requires the folding temperature to be identified based on the individual type of structure. Two different folded structures are fabricated and the protocol for identifying the annealing temperature is studied.