Ion induced ionization and fragmentation of biomolecules and biomolecular clusters

COFFEE_KLATCH · Invited

Abstract

The interaction of keV to MeV protons and heavy ions with DNA is the basis of ion therapy and risk assessment for manned space flight. The reason is, that in tissue the originally fast ions are decelerated to MeV energies and below (the Bragg peak region) where DNA damage is highest. In living cells, DNA damage always occurs in the presence of a chemical environment. The great complexity of such condensed systems rules out the investigation of fragmentation dynamics on an event by event level. For such studies, we need finite target systems still sufficiently complex to reflect relevant chemical aspects of the cell nucleus. We investigate ion collisions with isolated DNA building blocks and their neutral clusters to bridge the gap between isolated molecules and the condensed phase. In these studies, we could for instance show that intermolecular hydrogen bonds strongly affect the nucleobase fragmentation dynamics. Because of limitations of the cluster approach, we recently commissioned a novel setup interfacing an electrospray ion source with a keV ion beamline. This allows for generation of a protonated/deprotonated beam of virtually any biomolecule. The charged biomolecules are then collected and cooled in an RF trap and subsequently collided with keV ions. Time-of-flight mass spectrometry is used to study ionization and fragmentation dynamics. First results on small protonated peptides and DNA building blocks prove the great potential of the technique.

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Authors

  • Tug Yasar

    Theoretical Electrical Engineering, Ruhr-Universit\"at Bochum, Tokyo Electron AT LTD, Ruhr University Bochum, Pusan National University, Department of Electronic Engineering, Tohoku University, GE Historian, TET, AEPT, Nagoya Institute of Technology, Korean Institute of Energy Research, Department of Materials Science and Engineering, Kunsan National University, University of California at Berkeley, Dept. of Chemistry, Ajou University, GE Global Reseach, Niskayuna, US, TRINITI, Troizck, Russia, Kintech Lab, Moscow, Russia, HanYang University, West Virginia University, Morgantown, WV 26506, Air Force Research Laboratory, Wright-Patterson AFB, OH 45433, Norwegian University of Science and Technology (NTNU), University of Wisconsin-Madison, Institute for Theoretical Electrical Engineering, Ruhr University Bochum, Chubu University, NEC Corporation, Nagoya University, Universit\'e Paris-Sud, Dong-A University, Saga University, University of Ryukyus, Sasebo National College of Technology, Faculty of Stomatology, Belgrade, Serbia, Institute of Physics, Belgrade, Serbia, Seagate Technology, NIBEC, LPP, Ecole Polytechnique, France, State University, Saint-Petersburg, LPP, Ecole Polytechnique, Dept. of Physics, California State University, Fullerton, CA, Institute of High Performance Computing, Singapore, Dept. of Physics, UAE University, Abu Dhabi, United Arab Emirates, Dept. of Physics, Missouri University of Sci. \& Tech., Rolla, MO, Tsinghua University, Beijing, China, University of Adelaide, Australia, Missouri University of Science and Technology, M.M.H. College, Ghaziabad, The Open University, University of Kentucky, Department of Applied Physics, Hanyang University, Ansan, Kyunggi-Do 426-791, South Korea, Hiden Analytical Ltd, Warrington, UK, Queens University Belfast, UK, University of Nevada, Reno,USA, Stalder Technologies and Research, Redwood City, USA, Queen's University Belfast, Loras College, Auburn University, LBNL, University of Nebraska-Lincoln, University of Newcastle-Ourimbah, Pacific Union College, University of Manchester (UK), Los Alamos National Laboratory, IMEC, Leuven, Belgium, Nuclear Physics Institute, Moscow State University, Moscow, Russia, Kintech Lab Ltd, Tokyo Electron Ltd., Kyoto University, Zeon Corporation, CAMS, University of Adelaide, South Australia, University of North Texas, Denton, CAMS, Australian National University, Canberra, Drake University, University of Western Australia, Fraunhofer Institute IVV Freising, Ruhr-University Bochum, University of California, San Diego, CAMS, Flinders University, Adelaide, South Australia, Univ. Groningen, IBM T.J. Watson Research Center, Surfx Technologies, Cymer, Inc., Department of Physics, Florida A\&M University, Dept. of Physics, UAE University, Abu Dhabi, United Arab, Dept. of Physics, Missouri University of Science \& Technology, Rolla, Mo, Institute for Plasma and Atomic Physics, Ruhr-University Bochum, 44780 Bochum, Germany, Faculty of Physics, Sofia University, 5, J. Bourchier Blvd., BG-1164 Sofia, Bulgaria, Faculty of Science and Technology, Keio University, RRC Kurchatov Inst., Moscow, Russia, Keio University, Yokohama, Japan, Macquarie University, University of Windsor, Mattson Technology Inc, Plasma Dynamics, RF Plasma Consulting, James Cook University Townsville, Institute of Physics Belgrade, University of Alberta, Edmonton, Canada, Princeton Plasma Physics Laboratory, Princeton, NJ, USA, Department of Aeronautics and Astronautics, Kyoto University, GE Global Reseach, Niskayuna,US, IPFN - IST, Portugal, St. Kliment Ohridski University of Sofia, Texas A\&M Mechanical Engineering, B \& W Group Ltd, UK, Belgrade, Institute of Physics, NIBEC University of Ulster, Dep. Fis/UCO, Cordoba, Spain, IPFN/IST, Lisbon, Portugal, ICMSE/CSIC, Sevilla, Spain, St. Petersburg State University, Russia, LPGP/UPS, Orsay, France, Seoul National University, Daehak-dong, Kwanak-gu, Seoul, Korea, Hanyang University, Plasmetrex GmbH Berlin, LATMOS/UVSQ, Verrieres le Buisson, France, Hungarian Academy for Science, Plasma Physics Division, Naval Research Laboratory, Global Strategies Group (North America), Sandia National Laboratories, Lebedev Physics Institute, Moscow, Department of Physics, Kurdistan region, Duhok University, Iraq, SPbSU, AFRL, Instituto de Ciencias Fisicas, UNAM, UES Inc., 4401 Dayton-Xenia Rd., Beavercreek, OH 45322, USA, Seoul National University, Samsung Electronics Co., University of Illinois at Urbana Champaign, Queen`s University Belfast, GE Research Laboratory, US, TRINITI, Troitsk, Russia, Dept. of Math \& Physics, Troy University, CPP Queens University Belfast, Department of Electrical Engineering, Hanyang University, Nagoya University, JST-CREST, NIST, Katagiri Engineering Co., Ltd., NU System Co., Ltd., Meijo University, Wakayama University, NU EcoEngineering Co., Ltd., University of Kansas, Princeton University, The von Karman Institute for Fluid Dynamics, Varian Semiconductor Equipment Associates, Institute of Physics, Faculty of Transport and Traffic Engineering, VSEA, Center for Atomic and Molecular Technologies, Osaka University, Princeton Plasma Physics Laboratory, Tokyo Electron, US. Holdings