Dual Syringe Electrospinning of FNfds-modified Hyaluronic Acid

ORAL

Abstract

We described the fabrication of a unique HA nanofibrous scaffold using dual syringe reactive electrospinning. 3$^{'}$-dithiobis(propanoic dihydrazide)-modified HA (HA-DTPH) and Poly (ethylene glycol)-diacrylate (PEGDA) were selected as the cross-linking system. PEO was blended with HA-DTPH to facilitate the fiber formation. Fibronectin functional domains (FNfds) were incorporated with PEGDA and covalently linked to HA via conjugate addition to improve the cell attachment. The as-spun scaffold was soaked into DI water to remove PEO and yield an FNfds-modified HA-DTPH nanofibrous scaffold. Human dermal fibroblasts CF31 were seeded on FNfds-modified HA-DTPH scaffolds. The CF31 fibroblasts showed a unique extended dendritic morphology which is opposed to the typical flattened morphology of cells on regular 2D geometries. Supported by NSF-MRSEC.

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Authors

  • Yuan Ji

    Dept. of Materials Science, SUNY at Stony Brook

  • Kaustabh Ghosh

    Dept. of Biomedical Engr., SUNY at Stony Brook

  • Xiaozheng Shu

    Dept. of Medicinal Chemistry, the University of Utah

  • Jonathan Sokolov

    Dept. of Materials Science, SUNY at Stony Brook, Dept. Mat. Sci. \& Eng., SUNY at Stony Brook

  • Glenn Prestwich

    Dept. of Medicinal Chemistry, the University of Utah

  • Richard Clark

    BME Dept, SUNY Stony Brook, Dept. of Biomedical Engr., SUNY at Stony Brook

  • M. Rafailovich

    SUNY, Dept. of Materials Science, SUNY at Stony Brook, Department of Materials Science, SUNY at Stony Brook, Dept. of Mat. Sci. and Engr,, State Univ. of New York at Stony Brook, Stony Brook Univ Stony Brook NY