Continuous separation of serum from human whole blood within a microfluidic device

ORAL

Abstract

We were able to demonstrate separation of red and white blood cells from their native blood plasma, using a technique known as deterministic lateral displacement. The device takes advantage of asymmetric bifurcation of laminar flow around obstacles. This asymmetry creates a size dependent deterministic path through the device. All components of a given size follow equivalent migration paths, leading to high resolution. A subsequent version of the device will focus on the removal of platelets from whole blood. Samples will be extracted from the microfluidic device and analyzed by conventional flow cytometry.

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Authors

  • John Davis

    Princeton University

  • David Inglis

    Princeton University, Princeton Institute for the Science and Technology of Materials, Princeton University

  • James Sturm

    Princeton University, Princeton Institute for the Science and Technology of Materials, Princeton University

  • Robert Austin

    Princeton University, Princeton Universiy Dept. of Physics, Department of Physics, Princeton University