Single-Molecule Tracking of Polymers on Surfaces

ORAL

Abstract

Single-molecule tracking technique is, for the first time, applied to study adsorbed polymers on surfaces. Fluorescently-labeled poly (ethylene glycol) (PEG) chains are allowed to freely adsorb from dilute aqueous solutions to various surfaces with different affinity. After rinsing away any non-adsorbed chains, the motion of the surface-bound species is tracked using objective-based total internal reflection fluorescence microscopy (TIRFM). Using our single particle tracking algorithm, individual chain behaviors are analyzed with sub-diffraction resolution.

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Authors

  • Juan Guan

    U of Illinois Urbana-Champaign

  • Bo Wang

    U of Illinois Urbana-Champaign, Department of Materials Science and Engineering, University of Illinois

  • Stephen Anthony

    University of Illinois, U of Illinois Urbana-Champaign

  • Sung Chul Bae

    U of Illinois Urbana-Champaign, Department of Materials Science and Engineering, University of Illinois

  • Subhalakshmi Kumar

    U of Illinois Urbana-Champaign

  • Steve Granick

    University of Illinois, Department of Materials Science and Engineering, University of Illinois, University of Illinois, Department of Materials Science and Engineering, U of Illinois Urbana-Champaign, University of Illinois at Urbana Champaign, Department of Materials Science and Engineering, University of Illinois at Urbana-Champaign, University of Illinois, Urbana Champaign