Comparison anti-bacterial effect of silver/polystyrene nanocomposites on gram negative and positive bacteria

ORAL

Abstract

Silver nanoparticles/polystyrene nanocomposites were prepared via casting the solution of polystyrene in a mixture of carbon tetrachloride and acetone containing silver nanoparticles. Colloidal silver nanoparticles in acetone were synthesized by pulsed laser ablation (PLA) of pure bulk silver. Casting the colloidal silver nanoparticles in a solution of polystyrene results in a yellowish transparent polymeric sheet. TEM images show rather spherical nanoparticles with mean diameter of 5 nm. Ag/PS nanocomposites were characterized by UV-VIS spectroscopy. In this study, we also investigated the antimicrobial activity of silver nanocomposites against Escherichia coli (E.coli) and Staphylococcus aureus (S.aureus) as a model for Gram negative and Gram positive bacteria. Antibacterial tests were performed against E. coli and S.aureus, on LB agar plates containing different amount of nanoparticles. Our results showed at all these concentrations, the nanoparticles caused a growth delay of E. coli, increasing the concentration of nanoparticles increased this growth delay.

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Authors

  • Akhtarolmolook Kazemi

    Biochemical and Bioenvironmental Engineering Research Center, Sharif University of Technolog

  • Maryam Raftari

    The University of Sheffield, Western Bank, Sheffield S10 2TN, UK, Department of Physics and Astronomy, University of Sheffield, Sheffield S3 7RH, UK, Department of physics and Astronomy, University of Sheffield, Sheffield, UK

  • Sajjad Tollabimazraehno

    Johannes Kepler University, Altenberger Str. 69, 4040 Linz, Austria

  • Mohammad Mahdavi

    Sharif University of Technology, Azadi Avenue, Tehran 14588, Iran, Department of Physics, Sharif University of Technology

  • Azam Irajizad

    Institute for Nanoscience and nanotechnology Sharif University of Technology, Azadi Avenue, Tehran 14588, Iran, Institute for Nanoscience and nanotechnology, Sharif University of Technology