Formation of Lipid-Based Nanodiscs and Their Dependence of Temperature and Chemical Composition

ORAL

Abstract

Phospholipid mixtures composed of \textit{1,2-dipalmitoyl-sn-glycero-3-phosphocholine }(DPPC), \textit{1,2-dihexanoyl-sn-glycero-3-phosphocholine }(DHPC) and \textit{1,2-dipalmitoyl-sn-glycero-3-phospho-(1'-rac-glycerol) (sodium salt)}(DPPG) and\textit{ 1,2-distearoyl-sn-glycero-3-phosphoethanolamine-N-[methoxy(polyethylene glycol)-2000] (ammonium salt)} (PEGylated DSPE) and cholestrol were found to form nanodiscs (bicelles) in both non-ionic and phosphate buffer solutions . The structure of the aggregates is resolved using dynamic light scattering, transmission electron microscopy and small angle neutron scattering. The effects of temperature and chemical composition (e.g., PEGylated DSPE and cholesterol) on the structural variation and polydispersity will be discussed in this presentation. These nanodiscs have the potential of serving as a model delivery carrier for hydrophobic molecules for their biological compatibility and capability of incorporating with targeting molecules.

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Authors

  • Ying Liu

    UNIVESITY OF CONNECTICUT

  • Yongkun Yang

    UNIVESITY OF CONNECTICUT, University of Connecticut Polymer Program

  • Mu-Ping Nieh

    University of Connecticut, Institute of Materials Science, Chemical, Materials \& Biomolecular Engineering, UNIVESITY OF CONNECTICUT, University of Connecticut, Storrs, CT, USA, University of Connecticut Chemical Engineering/IMS, Department of Chemical, Materials \& Biomolecular Engineering, Institute of Materials Science, University of Connecticut, Storrs, CT 06269, USA, University of Connecticut, Institute of Materials Science, Chemical Materials \& Biomolecular Engineering, University of Connecticut