Simulations of Concentrated Antibody Solutions and Comparison with Small-Angle Scattering Experiments

POSTER

Abstract

We have performed atomically detailed Monte-Carlo simulations of hundreds of antibodies in concentrated solutions. In order to compare our simulations with experiments, we developed a novel method for explicitly calculating the scattering intensity of these large systems. At various salt conditions and pH levels, the simulations are found to be in good agreement with small-angle X-ray scattering measurements for antibody concentrations exceeding 100 mg/mL.

Authors

  • Max Watson

    National Institute of Standards and Technology

  • Nicholas Clark

    National Institute of Standards and Technology

  • Joseph Curtis

    National Institute of Standards and Technology