Lattice Light Sheet Microscopy of Endocytosis in Macrophages

Live cell, volumetric fluorescence imaging using a Lattice Light Sheet microscope, developed in collaboration with HHMI Janelia Farm Research Campus[1] is used to visualize endocytosis of B cells by macrophages derived from mouse bone marrow. Cells are gene edited to express fluorescence of specific organelles and/or labeled with fluorescent antibodies. Volumetric, multi-color fluorescence imaging reveals cellular level dynamical details of this process. Discussion of major findings, instrumentation, and data visualization will be presented[2].