Self-assembly and soluble aggregate behavior of computationally designed coiled-coil peptide bundles

ORAL

Abstract

Peptides are excellent candidates for nanomaterials design and controlled assembly due to their atomistic precision in designed structure formation as in biological systems. Unlike natural proteins and natural structural motifs, this effort is completely de novo in order to build arbitrary structures with desired size, shape and display of functional groups. We have successfully prepared soluble, coiled-coil, peptide tetramer bundles that are robust and stable. Using circular dichroism we demonstrated bundle thermal stability and confirmed their alpha helical and coiled-coil nature. The coiled-coil tetramer was confirmed by analytical ultra-centrifugation sedimentation studies. We also established how these bundles behave in solution using small angle neutron scattering. The form factor of the bundles is well represented by a cylinder model with dimensions consistent with the size of the designed bundles. At high concentrations, the behavior of the bundles is modeled using a structure factor for soluble aggregates with an average inter bundle separation. These experiments support our claim that the designed coiled-coil bundles were achieved in solution and interact in a way similar to natural proteins in concentrated solution.

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Presenters

  • Michael Haider

    Materials Science and Engineering, University of Delaware

Authors

  • Michael Haider

    Materials Science and Engineering, University of Delaware

  • Huixi Zhang

    Chemistry, University of Pennsylvania, Department of Chemistry, University of Pennsylvania

  • Nairiti Sinha

    Materials Science and Engineering, University of Delaware, Materials Science & Engineering, Univ of Delaware

  • Kristi Kiick

    Materials Science and Engineering, University of Delaware, Materials Science and Engineering Department, University of Delaware

  • Jeffery Saven

    Chemistry, University of Pennsylvania, Department of Chemistry, University of Pennsylvania, University of Pennsylvania

  • Darrin Pochan

    Univ of Delaware, Materials Science and Engineering, University of Delaware, Materials Science and Engineering Department, University of Delaware, Materials Science and Engineering, Univ of Delaware, Materials Science & Engineering, Univ of Delaware