Array atomic force microscopy for real-time multi-parametric analysis

Nanoscale multipoint structure-function analysis is essential for deciphering complexity of multiscale biological and physical system. Here, we describe a prototype of dispersive optics-based array AFM capable of simultaneously monitoring multiple probe-sample interactions in air and in liquid. A single supercontinuum laser beam is utilized to spatially and spectrally map multiple cantilevers, so that the beam deflection from individual cantilever can be isolated and recorded by distinct wavelength selection. This new design provides a remarkably simplified yet effective solution to overcome optical crosstalk, while maintaining high sensitivity and compatibility with probe-based sensors. We demonstrate the versatility and robustness of our system on parallel multi-parametric imaging, as well as monitoring live hearts cells intercellular activity. This approach provides new opportunities for studying emergent properties of atomic-scale mechanical and physicochemical interactions in a wide range of biological and physical networks.