Scanning Electron Microscopy – New Approaches in Imaging Biological Specimens

Scanning Electron Microscopy (SEM) of biological systems has a long and illustrious history. However due to the inherent complexities of biological hard and soft matter interaction with electrons and with the imaging system, one must overcome certain hurdles. We have attempted to optimize typical biological SEM sample preparation such as critical point drying, fixing of microbes via Osmium Tetroxide and other techniques in this investigation. In the study, we have also focused on the effect of the conductive coating (magnetron sputter deposited) on the specimen. The study concluded with stereoscopic 3D imaging of the specimen acquired by the SEM Secondary Electron and Backscattered Electron Detectors. We will discuss the results and compare them with the current literature.