Anti-cancer drug containing apolioprotein B lipoparticle reconstitution and tracking in living cell

ORAL

Abstract

Radicicol (Rad), an Hsp 90 inhibitor, is an antitumor drug which loses its antitumor activity during delivery process, in vivo. By adding Rad in the reconstituting apolioprotein B (apoB) process, a Rad containing reconstituted apoB lipoparticle (rABL) complex (Rad@rABL) can be reconstituted and characterized by photonic spectroscopies, differential scanning calorimetry and super resolution imaging. Interestingly, the Rad@rABL single molecule can be tracked within the living cell. The drug delivery route to the mitochondria in the Hep G2 cell line can be monitored. Moreover, the anticancer efficacy of Rad can be enhanced. In summary, rABL is an excellent and native self-assembly drugs carrier and living cell tracking agent.

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Presenters

  • Wei-Ping Chang

    Department of Biological Science and Technology, National Chiao Tung University,Taiwan

Authors

  • Wei-Ping Chang

    Department of Biological Science and Technology, National Chiao Tung University,Taiwan

  • Chung Ching Lin

    Department of Biological Science and Technology, National Chiao Tung University,Taiwan

  • Hsueh-Liang Chu

    Department of Biological Science and Technology, National Chiao Tung University,Taiwan

  • Po-Yen Lin

    Academia Sinica, Taiwan, Division of Core Facilities Imaging, Institute of Cellular and Organismic Biology, Academia Sinica, Taiwan

  • Yu-Chuan Liang

    Agricultural Biotechnology Research Center, Academia Sinica, Taiwan.

  • Caleb G. Chen

    Department of Hematology-Oncology, Mackay Memorial Hospital, Taiwan

  • Chia-Ching Chang

    Biological Science and Technology, National Chiao Tung University, Department of Biological Science and Technology, National Chiao Tung University,Taiwan, Department of Biological Science and Technology, National Chiao Tung University，Taiwan