LiveFISH: Visualizing endogenous RNA in living cells

RNA takes part in genome regulation dynamically in living cells. However, studying RNA molecules in action is difficult. Here we present a technique by which endogenous RNA molecules may be visualized directly in living mammalian cells. Our approach (liveFISH), based on fluorescence in situ hybridization (FISH) but extended to work in living cells, overcomes previous limitations, and is capable of labeling arbitrary species of endogenous RNA without need for genetic modification.