DNA Local Flexibility Dependent Formation of Liquid-like Droplets

Associative phase separation of biomacromolecules like proteins and nucleic acids into liquid-like droplets, termed liquid-liquid phase separation (LLPS), has been linked to sans lipid-membrane intracellular organization as well as aggregation mediated diseases. In this work, we have studied the role of sequence-dependent DNA flexibility in LLPS of multiple interacting components. We find that DNA local flexibility, encoded by the DNA sequence, not simply the overall charge density, determines the phase behavior in such systems.