Performing Isothermal Molecular Amplification in Nanocavities with Tunable Confinement
ORAL
Abstract
Nanofluidic devices featuring real time-confinement control enable capture, isolation and biochemical processing of single analytes in nanoscale cavities for highly parallel and individualized single-particle/single-molecule analysis. This technology may find a range of applications ranging from analysis of single extracellular vesicles to digital nucleic acid tests. Here, using a device approach based on pneumatic actuation of a thin membrane lid to seal an embedded nanocavity in a nanoslit, we demonstrate key steps necessary for single cavity molecular amplification. These steps include cavity loading by target nucleic acids, exposure of the loaded cavity to required enzymes/co-factors and then implementation of an isothermal amplification reaction via Loop-mediated Isothermal AMPlification (LAMP) and rolling circle (RCA) approaches.
* The authors thank the Canadian Cancer Society (255878 CCSRI), Natural Science and Engineering Research Council of Canada (NSERC, G247765), New Frontiers for Research Fundexploration (250326 NFRFE) and Canada Foundation for Innovation (CFI, G248924) for financial support. The authors acknowledge Nanotools-Microfab at McGill University and INRS Varennes. I.I.M. acknowledge Faculty of Engineering at McGill University for MEDA award and CMC for the financial support.
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Presenters
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Walter W Reisner
McGill Univ, McGill University, Department of Physics
Authors
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Walter W Reisner
McGill Univ, McGill University, Department of Physics
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Imman I Hosseini
McGill University, Department of Biomedical Engineering
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Vahid Hamidi
McGill University, Department of Biomedical Engineering
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Zezhou Liu
McGill University, Department of Physics
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Xavier Capaldi
McGill University, Dept. of Physics
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Sara Mahshid
McGill University, McGill University, Department of Biomedical Engineering