Vibrational Fingerprints of Alkaline Phosphatase (ALP): An in-situ FTIR Study

ORAL

Abstract

Alkaline phosphatase (ALP), a fundamental player in a wide array of vital biological processes, holds immense significance for researchers in the realms of both basic and applied sciences. Here we use Fourier-transform infrared spectroscopy (FTIR) to characterize the spectra and molecular vibrations of ALP, and p-nitrophenyl phosphate (PNPP) into p-nitrophenol (PNP) while subjecting this process to varying ALP concentrations. We will discuss the role of relevant vibrations throughout the chemical reaction and their evolution during the distinct stage of it. These findings offer new insights into the intricate molecular interactions of ALP and its functionality.

* We would like to thank Gordon and Betty Moore Foundation's EPiQS Initiative Experimental Investigator Award, Grant ID GBMF9464.

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Presenters

  • Haoyue Jiang

    UC Berkeley

Authors

  • Haoyue Jiang

    UC Berkeley

  • Shannon Yan

    University of California, Berkeley

  • Liana Klivansky

    Lawrence Berkeley National Laboratory

  • Zhijie Chen

    BridgeBio

  • Carlos J Bustamante

    University of California, Berkeley

  • Alessandra Lanzara

    University of California, Berkeley

  • Luca Moreschini

    University of California, Berkeley