In vivo protein dynamics

Protein dynamics in cells is subject to both physical and evolutionary constraints. I will discuss a series of experiments and simulations to highlight how protein dynamics is modulated in its native cytoplasmic environment, and how it differs from similar dynamics in aqueous solution. Examples will include the activation of chaperones in the cytoplasm, in-situ protein folding dynamics inside live cells and animals, resolving the dwell times and transition state passage times for protein large-amplitude conformational changes, as well as resolving in-cell dynamics of protein complexes such as microtubules or spliceosomal components.