Liposomes and Sonoporation

A method to prepare liposomes is presented. Liposomes made in our laboratory were characterized acoustically and optically. The phase velocity and attenuation of liposomes in suspension (concentration = 10$^{9}$/mL) were measured ranging from 2 to 14 MHz using ultrasound spectroscopy. Using the liposomes, anti rabbit IgG conjugated with Alexafluor 647 was delivered into Jurkat cells in suspension by 10 {\%} duty cycle ultrasound tonebursts of 2.2 MHz (the \textit{in situ} spatial peak-pressure amplitude = 80W/cm$^{2})$ with an efficiency of 13 {\%}. It has been experimentally shown liposomes may be an alternative stable agent to Optison$^{\mbox{{\textregistered}}}$ for delivering macromolecules into cells.