Dye-Enhanced Multimodal Confocal Imaging of Brain Cancers

ORAL

Abstract

\textbf{Background and Significance}: Accurate high resolution intraoperative detection of brain tumors may result in improved patient survival and better quality of life. The goal of this study was to evaluate dye enhanced multimodal confocal imaging for discriminating normal and cancerous brain tissue. \textbf{Materials and Methods}: Fresh thick brain specimens were obtained from the surgeries. Normal and cancer tissues were investigated. Samples were stained in methylene blue and imaged. Reflectance and fluorescence signals were excited at 658nm. Fluorescence emission and polarization were registered from 670 nm to 710 nm.~The system provided lateral resolution of 0.6 $\mu $m and axial resolution of 7 $\mu $m. Normal and cancer specimens exhibited distinctively different characteristics. H{\&}E histopathology was processed from each imaged sample. \textbf{Results and Conclusions}: The analysis of normal and cancerous tissues indicated clear differences in appearance in both the reflectance and fluorescence responses. These results confirm the feasibility of multimodal confocal imaging for intraoperative detection of small cancer nests and cells.

–

Authors

  • Dennis Wirth

    University of Massachusetts Lowell

  • Richard D. Averitt

    Trinity College, Department of Physics, Joint appointment in Teacher Education, Wright State University, Graduate School of Education, University of Massachusetts Lowell, Littleton High School, Department of Physics, Boston College, Department of Physics and Applied Physics, University of Massachusetts Lowell, North Carolina State University, University of Massachusetts at Lowell, University of Massachusetts Lowell, Harvard Medical School, Massachusetts General Hospital, Boston, MA, Harvard University, Department of Chemistry University of Massachusetts Amherst, Center for Advanced Materials and Department of Plastics Engineering University of Massachusetts Lowell, Center for Advanced Materials and Department of Physics and Applied Physics University of Massachusetts Lowell, RMD Inc., Watertown MA, UMass Lowell, Lowell MA, Radiation Monitoring Devices Inc., Watertown, MA, USA, University of Massachusetts, Lowell, MA, USA, Argonne National Laboratory, Argonne, IL, USA, ANL, IL, UMass Lowell, Brown University / Department of Chemistry, Sciprint.org, Physics Department, LESIMS Laboratory, Badji Mokhtar University, 23000 Annaba, Algeria, Department of Materials Science and Engineering, Massachusetts Institute of Technology, Physics Department, Boston University

  • Richard D. Averitt

    Trinity College, Department of Physics, Joint appointment in Teacher Education, Wright State University, Graduate School of Education, University of Massachusetts Lowell, Littleton High School, Department of Physics, Boston College, Department of Physics and Applied Physics, University of Massachusetts Lowell, North Carolina State University, University of Massachusetts at Lowell, University of Massachusetts Lowell, Harvard Medical School, Massachusetts General Hospital, Boston, MA, Harvard University, Department of Chemistry University of Massachusetts Amherst, Center for Advanced Materials and Department of Plastics Engineering University of Massachusetts Lowell, Center for Advanced Materials and Department of Physics and Applied Physics University of Massachusetts Lowell, RMD Inc., Watertown MA, UMass Lowell, Lowell MA, Radiation Monitoring Devices Inc., Watertown, MA, USA, University of Massachusetts, Lowell, MA, USA, Argonne National Laboratory, Argonne, IL, USA, ANL, IL, UMass Lowell, Brown University / Department of Chemistry, Sciprint.org, Physics Department, LESIMS Laboratory, Badji Mokhtar University, 23000 Annaba, Algeria, Department of Materials Science and Engineering, Massachusetts Institute of Technology, Physics Department, Boston University

  • Richard D. Averitt

    Trinity College, Department of Physics, Joint appointment in Teacher Education, Wright State University, Graduate School of Education, University of Massachusetts Lowell, Littleton High School, Department of Physics, Boston College, Department of Physics and Applied Physics, University of Massachusetts Lowell, North Carolina State University, University of Massachusetts at Lowell, University of Massachusetts Lowell, Harvard Medical School, Massachusetts General Hospital, Boston, MA, Harvard University, Department of Chemistry University of Massachusetts Amherst, Center for Advanced Materials and Department of Plastics Engineering University of Massachusetts Lowell, Center for Advanced Materials and Department of Physics and Applied Physics University of Massachusetts Lowell, RMD Inc., Watertown MA, UMass Lowell, Lowell MA, Radiation Monitoring Devices Inc., Watertown, MA, USA, University of Massachusetts, Lowell, MA, USA, Argonne National Laboratory, Argonne, IL, USA, ANL, IL, UMass Lowell, Brown University / Department of Chemistry, Sciprint.org, Physics Department, LESIMS Laboratory, Badji Mokhtar University, 23000 Annaba, Algeria, Department of Materials Science and Engineering, Massachusetts Institute of Technology, Physics Department, Boston University

  • Richard D. Averitt

    Trinity College, Department of Physics, Joint appointment in Teacher Education, Wright State University, Graduate School of Education, University of Massachusetts Lowell, Littleton High School, Department of Physics, Boston College, Department of Physics and Applied Physics, University of Massachusetts Lowell, North Carolina State University, University of Massachusetts at Lowell, University of Massachusetts Lowell, Harvard Medical School, Massachusetts General Hospital, Boston, MA, Harvard University, Department of Chemistry University of Massachusetts Amherst, Center for Advanced Materials and Department of Plastics Engineering University of Massachusetts Lowell, Center for Advanced Materials and Department of Physics and Applied Physics University of Massachusetts Lowell, RMD Inc., Watertown MA, UMass Lowell, Lowell MA, Radiation Monitoring Devices Inc., Watertown, MA, USA, University of Massachusetts, Lowell, MA, USA, Argonne National Laboratory, Argonne, IL, USA, ANL, IL, UMass Lowell, Brown University / Department of Chemistry, Sciprint.org, Physics Department, LESIMS Laboratory, Badji Mokhtar University, 23000 Annaba, Algeria, Department of Materials Science and Engineering, Massachusetts Institute of Technology, Physics Department, Boston University