Mitigating radiation damage in x-ray macromolecular crystallography at synchrotron sources

ORAL

Abstract

A new strategy is presented to reduce primary x-ray damage in macromolecular crystallography using synchrotron radiation. The basic principle was to separate as much as possible the x-ray irradiated region, where the diffracted signal originates, from the region where damage accumulates. This is possible since, by far, most of the damage is caused by the photo-electrons (PE's) excited as an x-ray photon is absorbed, and distributes its damage typically over several $\mu $m's. The optimum method to accomplish this was to focus the x-rays to a vertical line of sub-$\mu $m width. Diffraction experiments performed recently at the Advanced Photon Source, with a 0.83x60$\mu $m vertical line focus, allowed us to directly measure the spatial dependence of radiation damage in lysozyme crystals. The PE's caused radiation damage to accumulate predominatly outside the irradiateded region of the crystal exposed with line focused beam leading to a 4.5 factor (450{\%}) decease of radiation damage in the focused diffraction signal.

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Authors

  • A. Knecht

    Oregon State University, University of Idaho, Department of Chemistry, Oregon State University, Corvallis, OR, School of EECS, Oregon State University, Corvallis, OR, National Renewable Energy Laboratory, Golden, CO, Oregon Health and Science University, Linfield College, Purdue University, National Institute of Standards and Technology, Montana State University, University of British Columbia, University of Washington, Cornell University, Philipps-University, 35032 Marburg, Germany, University of Arizona, Tucson, Arizona 85721, Oregon State University, Corvallis, OR 97331, Physics Department, SUNY Binghamton, Physics Department, Oregon State University, Argonne National Laboratory

  • A. Knecht

    Oregon State University, University of Idaho, Department of Chemistry, Oregon State University, Corvallis, OR, School of EECS, Oregon State University, Corvallis, OR, National Renewable Energy Laboratory, Golden, CO, Oregon Health and Science University, Linfield College, Purdue University, National Institute of Standards and Technology, Montana State University, University of British Columbia, University of Washington, Cornell University, Philipps-University, 35032 Marburg, Germany, University of Arizona, Tucson, Arizona 85721, Oregon State University, Corvallis, OR 97331, Physics Department, SUNY Binghamton, Physics Department, Oregon State University, Argonne National Laboratory

  • Y. Zou Finfrock

  • A. Knecht

    Oregon State University, University of Idaho, Department of Chemistry, Oregon State University, Corvallis, OR, School of EECS, Oregon State University, Corvallis, OR, National Renewable Energy Laboratory, Golden, CO, Oregon Health and Science University, Linfield College, Purdue University, National Institute of Standards and Technology, Montana State University, University of British Columbia, University of Washington, Cornell University, Philipps-University, 35032 Marburg, Germany, University of Arizona, Tucson, Arizona 85721, Oregon State University, Corvallis, OR 97331, Physics Department, SUNY Binghamton, Physics Department, Oregon State University, Argonne National Laboratory

  • A. Knecht

    Oregon State University, University of Idaho, Department of Chemistry, Oregon State University, Corvallis, OR, School of EECS, Oregon State University, Corvallis, OR, National Renewable Energy Laboratory, Golden, CO, Oregon Health and Science University, Linfield College, Purdue University, National Institute of Standards and Technology, Montana State University, University of British Columbia, University of Washington, Cornell University, Philipps-University, 35032 Marburg, Germany, University of Arizona, Tucson, Arizona 85721, Oregon State University, Corvallis, OR 97331, Physics Department, SUNY Binghamton, Physics Department, Oregon State University, Argonne National Laboratory

  • A. Knecht

    Oregon State University, University of Idaho, Department of Chemistry, Oregon State University, Corvallis, OR, School of EECS, Oregon State University, Corvallis, OR, National Renewable Energy Laboratory, Golden, CO, Oregon Health and Science University, Linfield College, Purdue University, National Institute of Standards and Technology, Montana State University, University of British Columbia, University of Washington, Cornell University, Philipps-University, 35032 Marburg, Germany, University of Arizona, Tucson, Arizona 85721, Oregon State University, Corvallis, OR 97331, Physics Department, SUNY Binghamton, Physics Department, Oregon State University, Argonne National Laboratory

  • A. Knecht

    Oregon State University, University of Idaho, Department of Chemistry, Oregon State University, Corvallis, OR, School of EECS, Oregon State University, Corvallis, OR, National Renewable Energy Laboratory, Golden, CO, Oregon Health and Science University, Linfield College, Purdue University, National Institute of Standards and Technology, Montana State University, University of British Columbia, University of Washington, Cornell University, Philipps-University, 35032 Marburg, Germany, University of Arizona, Tucson, Arizona 85721, Oregon State University, Corvallis, OR 97331, Physics Department, SUNY Binghamton, Physics Department, Oregon State University, Argonne National Laboratory

  • A. Knecht

    Oregon State University, University of Idaho, Department of Chemistry, Oregon State University, Corvallis, OR, School of EECS, Oregon State University, Corvallis, OR, National Renewable Energy Laboratory, Golden, CO, Oregon Health and Science University, Linfield College, Purdue University, National Institute of Standards and Technology, Montana State University, University of British Columbia, University of Washington, Cornell University, Philipps-University, 35032 Marburg, Germany, University of Arizona, Tucson, Arizona 85721, Oregon State University, Corvallis, OR 97331, Physics Department, SUNY Binghamton, Physics Department, Oregon State University, Argonne National Laboratory

  • A. Knecht

    Oregon State University, University of Idaho, Department of Chemistry, Oregon State University, Corvallis, OR, School of EECS, Oregon State University, Corvallis, OR, National Renewable Energy Laboratory, Golden, CO, Oregon Health and Science University, Linfield College, Purdue University, National Institute of Standards and Technology, Montana State University, University of British Columbia, University of Washington, Cornell University, Philipps-University, 35032 Marburg, Germany, University of Arizona, Tucson, Arizona 85721, Oregon State University, Corvallis, OR 97331, Physics Department, SUNY Binghamton, Physics Department, Oregon State University, Argonne National Laboratory