Using spatial deconvolution to improve image contrast in a capillary-based high-pressure chamber for fluorescence microscopy

POSTER

Abstract

A quartz capillary is used as a simple-to-construct high-pressure chamber for fluorescence microscopy imaging, with capillary walls acting as both the mechanical support and optical window. Because wall curvature introduces image aberrations, the effectiveness of spatial deconvolution as an image restoration technique is investigated. The point-spread-function is measured in both a capillary and a flat-slide control, then applied in the imaging of sub-cellular dimension microspheres. Results demonstrate that sub-cellular dimension objects can be spatially resolved in a capillary capable of pressurization to several hundred atmospheres, the range of interest when studying pressure effects in cellular systems. Contrast improvement is observed for both high- and low-numerical-aperture objectives.

Authors

  • William H. Rauckhorst

    Gothenburg University, Denison University, Bowling Green State University, NASA Glenn Research Center, Ohio Wesleyan University, Nitronex Corporation, 2305 Presidential Drive, Durham, NC, AFRL/MLPS, Wright-Patterson AFB, OH, Miami University, Ursinus College, Purdue University Calumet, GSI, Florida State University, Carnegie Mellon University, Dept. of Health Sciences, Cleveland State University, Physics Dept., Cleveland State University, University of Rochester, OMI College of Applied Science, University of Cincinnati, Davis and Elkins College, WV, Schlumberger-Doll, Korea Institute of Science and Technology, University of Cincinnati, Lock Haven University of Pennsylvania, Oak Ridge National Laboratory, Oak Ridge, TN 37831-6372, Albion College, Albion, MI 49224, University of Toledo, Toledo, OH 43606, Youngstown State University, Joint Institute for Nuclear Research (Dubna), Naval Research Laboratory, ManTech SRS Technologies, Inc., Ecopulse, Inc., Army Research Laboratory, University of Surrey, The Ohio State University, Univ. Akron, Owens Community College, Toledo, OH

  • William H. Rauckhorst

    Gothenburg University, Denison University, Bowling Green State University, NASA Glenn Research Center, Ohio Wesleyan University, Nitronex Corporation, 2305 Presidential Drive, Durham, NC, AFRL/MLPS, Wright-Patterson AFB, OH, Miami University, Ursinus College, Purdue University Calumet, GSI, Florida State University, Carnegie Mellon University, Dept. of Health Sciences, Cleveland State University, Physics Dept., Cleveland State University, University of Rochester, OMI College of Applied Science, University of Cincinnati, Davis and Elkins College, WV, Schlumberger-Doll, Korea Institute of Science and Technology, University of Cincinnati, Lock Haven University of Pennsylvania, Oak Ridge National Laboratory, Oak Ridge, TN 37831-6372, Albion College, Albion, MI 49224, University of Toledo, Toledo, OH 43606, Youngstown State University, Joint Institute for Nuclear Research (Dubna), Naval Research Laboratory, ManTech SRS Technologies, Inc., Ecopulse, Inc., Army Research Laboratory, University of Surrey, The Ohio State University, Univ. Akron, Owens Community College, Toledo, OH

  • Paul Urayama

    Miami University