Pressure effects on the solvent denaturation of NADH

POSTER

Abstract

Nicotinamide adenine dinucleotide (NADH) plays a central role in cellular metabolism as an electron donor via an NAD+/NADH redox reaction. NADH conformational state -- whether it is folded or unfolded -- has physiological significance because it takes on an unfolded conformation when bound to proteins. This study examines the effects of pressure on the solvent denaturation of NADH (20$\mu $M NADH in MOPS buffer solution, pH7.4). Using a quartz capillary-based high-pressure chamber, the folding/unfolding transition of NADH was examined through excited-state emission spectroscopy (337 nm excitation) at physiological pressures up to 50 MPa. By using a two-state solvent-denaturation model to determine thermodynamic parameters of solvent denaturation and by using an Arrhenius relationship, the change in volume for the folding-unfolding reaction is determined.

Authors

  • Erik Alquist

    Miami University

  • Lauren Regueyra

    Miami University

  • Kurt Wiesenfeld

    Miami University, Summa Health System, Akron, John Carroll University, Prof, Dr, BfS, Germany, Florida State University, Monmouth College, Ohio Wesleyan University, Kenyon College, University of Cincinnati, Brookhaven National Lab, University of Wisconsin Oshkosh, Dept. of Chermical Engineering, Carnegie Mellon University, Cleveland State University, The Neurological Institute, Epilepsy Center, Department of Neurology, Cleveland Clinic, Un. of Stockholm, The University of Akron, Case Western Reserve University, West Virginia University, Kalamazoo College and Editor, American Journal of Physics, Denison University, University of Southern Florida, Johannes-Gutenberg-Universitat, BfS (Germany), Shanghai Jiao Tong University, Department of Physics, West Virginia University, Kansas State University, The Pennsylvania State University, University of Wisconsin-Oshkosh, Purdue University, Saint Jospeh's College, University of Washington, Indiana University, University of Potsdam, Georgia Institute of Technology

  • Kurt Wiesenfeld

    Miami University, Summa Health System, Akron, John Carroll University, Prof, Dr, BfS, Germany, Florida State University, Monmouth College, Ohio Wesleyan University, Kenyon College, University of Cincinnati, Brookhaven National Lab, University of Wisconsin Oshkosh, Dept. of Chermical Engineering, Carnegie Mellon University, Cleveland State University, The Neurological Institute, Epilepsy Center, Department of Neurology, Cleveland Clinic, Un. of Stockholm, The University of Akron, Case Western Reserve University, West Virginia University, Kalamazoo College and Editor, American Journal of Physics, Denison University, University of Southern Florida, Johannes-Gutenberg-Universitat, BfS (Germany), Shanghai Jiao Tong University, Department of Physics, West Virginia University, Kansas State University, The Pennsylvania State University, University of Wisconsin-Oshkosh, Purdue University, Saint Jospeh's College, University of Washington, Indiana University, University of Potsdam, Georgia Institute of Technology

  • Paul Urayama

    Miami University