Spot Surface Labeling of Magnetic Microbeads and Application in Biological Force Measurements

ORAL

Abstract

Biological force measurements on single molecules and macromolecular structures often use microbeads for the application of force. These techniques are often complicated by multiple attachments and nonspecific binding. In one set of experiments, we are applying a magnetic force microscope that allows us to pull on magnetic beads attached to ciliated human bronchial epithelial cells. These experiments provide a means to measure the stall force of cilia and understand how cilia propel fluids. However, because we are using beads with diameters of one and 2.8 microns, and the diameter of human airway cilia is approximately 200 nm, we cannot be assured that the bead is bound to a single cilium. To address this, we have developed a sputter coating technique to block the biotin binding capability of the streptavidin labeled bead over its entire surface except for a small spot. These beads may also have applications in other biological experiments such as DNA force experiments in which binding of a single target to an individual bead is critical.

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Authors

  • Ashley Estes

  • J. Deng

    US Naval Observatory, University of Virginia, Thomas Jefferson National Accelerator Facility, College of William and Mary, US Army Research Office, Brimrose Corporation of America, Hampton University, Department of Physics, University of Rajshahi, Rajshahi, Bangladesh, Department of Physics, Old Dominion University, Norfolk, VA 23529, University of North Carolina at Chapel Hill, Department of Physics, Old Dominion University, Norfolk, Virginia, Department of Physics, Korea University, Seoul, Korea, Department of Physics, University of Virginia, Charlottesville, VA, Department of Physics, Old Dominion University, Norfolk, VA, North Carolina State University and WebAssign, Raleigh, NC, North Carolina State University, Raleigh, NC, Broughton High School, Raleigh, NC, University of Wisconsin at Whitewater, Whitewater, WI, University of North Carolina-Chapel Hill Department of Physics, University of West Virginia - Chemistry Dept., ORNL, Old Dominion University, University of West Virginia Department of Chemistry, Clemson University, South Carolina Governor’s School for Science and Mathematics, College of Charleston, Rice University, Korea Research Institute of Standards and Science, Ohio State University, Wright State University, Yale U, JINR, Dubna, Univ. Fed. do Rio de Janeiro, Vanderbilt Univ./JIHIR(ORNL)/Tsinghua Univ., NSCL(MSU), LLNL, LBNL, Vanderbilt Univ./LBNL/JIHIR(ORNL), Vanderbilt Univ., Jagellonian University, University of Bonn, North Carolina A{\&T} State University, North Carolina Central University, Duke University and TUNL

  • J. Deng

    US Naval Observatory, University of Virginia, Thomas Jefferson National Accelerator Facility, College of William and Mary, US Army Research Office, Brimrose Corporation of America, Hampton University, Department of Physics, University of Rajshahi, Rajshahi, Bangladesh, Department of Physics, Old Dominion University, Norfolk, VA 23529, University of North Carolina at Chapel Hill, Department of Physics, Old Dominion University, Norfolk, Virginia, Department of Physics, Korea University, Seoul, Korea, Department of Physics, University of Virginia, Charlottesville, VA, Department of Physics, Old Dominion University, Norfolk, VA, North Carolina State University and WebAssign, Raleigh, NC, North Carolina State University, Raleigh, NC, Broughton High School, Raleigh, NC, University of Wisconsin at Whitewater, Whitewater, WI, University of North Carolina-Chapel Hill Department of Physics, University of West Virginia - Chemistry Dept., ORNL, Old Dominion University, University of West Virginia Department of Chemistry, Clemson University, South Carolina Governor’s School for Science and Mathematics, College of Charleston, Rice University, Korea Research Institute of Standards and Science, Ohio State University, Wright State University, Yale U, JINR, Dubna, Univ. Fed. do Rio de Janeiro, Vanderbilt Univ./JIHIR(ORNL)/Tsinghua Univ., NSCL(MSU), LLNL, LBNL, Vanderbilt Univ./LBNL/JIHIR(ORNL), Vanderbilt Univ., Jagellonian University, University of Bonn, North Carolina A{\&T} State University, North Carolina Central University, Duke University and TUNL

  • J. Deng

    US Naval Observatory, University of Virginia, Thomas Jefferson National Accelerator Facility, College of William and Mary, US Army Research Office, Brimrose Corporation of America, Hampton University, Department of Physics, University of Rajshahi, Rajshahi, Bangladesh, Department of Physics, Old Dominion University, Norfolk, VA 23529, University of North Carolina at Chapel Hill, Department of Physics, Old Dominion University, Norfolk, Virginia, Department of Physics, Korea University, Seoul, Korea, Department of Physics, University of Virginia, Charlottesville, VA, Department of Physics, Old Dominion University, Norfolk, VA, North Carolina State University and WebAssign, Raleigh, NC, North Carolina State University, Raleigh, NC, Broughton High School, Raleigh, NC, University of Wisconsin at Whitewater, Whitewater, WI, University of North Carolina-Chapel Hill Department of Physics, University of West Virginia - Chemistry Dept., ORNL, Old Dominion University, University of West Virginia Department of Chemistry, Clemson University, South Carolina Governor’s School for Science and Mathematics, College of Charleston, Rice University, Korea Research Institute of Standards and Science, Ohio State University, Wright State University, Yale U, JINR, Dubna, Univ. Fed. do Rio de Janeiro, Vanderbilt Univ./JIHIR(ORNL)/Tsinghua Univ., NSCL(MSU), LLNL, LBNL, Vanderbilt Univ./LBNL/JIHIR(ORNL), Vanderbilt Univ., Jagellonian University, University of Bonn, North Carolina A{\&T} State University, North Carolina Central University, Duke University and TUNL