Driven Microbead Rheology of Fibrin Gels

POSTER

Abstract

The rheological properties of fibrin, the primary structural element in blood clots, have been widely studied at the macroscopic level, because its mechanical properties are critical to its physiological function. Microbead rheology (MBR) shows promise for advancing this field in various ways. First, MBR can be performed on small sample quantities ($\sim $1 uL), which is useful for high-throughput experimentation; second, fibrin's complex structure has a range of length scales, such that large cells may not propagate while small viruses diffuse easily through the mesh. Microbeads from 10 um to under 500 nm can probe these length scales. These characteristics suggest MBR could be useful in screening drugs for disorders involving variant clot rigidity. We report on efforts to measure the rheology of fibrin gels over the course of its polymerization. A magnetic force microscope applies pulsed forces to microbeads suspended in fibrin gels. Beads are monitored on an inverted microscope and their positions tracked by software over the 30-minute course of the gelation. A single mode Jefferies model is used to extract viscosity and elasticity from the beads' creep-recovery.

Authors

  • J. Deng

    US Naval Observatory, University of Virginia, Thomas Jefferson National Accelerator Facility, College of William and Mary, US Army Research Office, Brimrose Corporation of America, Hampton University, Department of Physics, University of Rajshahi, Rajshahi, Bangladesh, Department of Physics, Old Dominion University, Norfolk, VA 23529, University of North Carolina at Chapel Hill, Department of Physics, Old Dominion University, Norfolk, Virginia, Department of Physics, Korea University, Seoul, Korea, Department of Physics, University of Virginia, Charlottesville, VA, Department of Physics, Old Dominion University, Norfolk, VA, North Carolina State University and WebAssign, Raleigh, NC, North Carolina State University, Raleigh, NC, Broughton High School, Raleigh, NC, University of Wisconsin at Whitewater, Whitewater, WI, University of North Carolina-Chapel Hill Department of Physics, University of West Virginia - Chemistry Dept., ORNL, Old Dominion University, University of West Virginia Department of Chemistry, Clemson University, South Carolina Governor’s School for Science and Mathematics, College of Charleston, Rice University, Korea Research Institute of Standards and Science, Ohio State University, Wright State University, Yale U, JINR, Dubna, Univ. Fed. do Rio de Janeiro, Vanderbilt Univ./JIHIR(ORNL)/Tsinghua Univ., NSCL(MSU), LLNL, LBNL, Vanderbilt Univ./LBNL/JIHIR(ORNL), Vanderbilt Univ., Jagellonian University, University of Bonn, North Carolina A{\&T} State University, North Carolina Central University, Duke University and TUNL

  • R.C. Spero

  • J. Deng

    US Naval Observatory, University of Virginia, Thomas Jefferson National Accelerator Facility, College of William and Mary, US Army Research Office, Brimrose Corporation of America, Hampton University, Department of Physics, University of Rajshahi, Rajshahi, Bangladesh, Department of Physics, Old Dominion University, Norfolk, VA 23529, University of North Carolina at Chapel Hill, Department of Physics, Old Dominion University, Norfolk, Virginia, Department of Physics, Korea University, Seoul, Korea, Department of Physics, University of Virginia, Charlottesville, VA, Department of Physics, Old Dominion University, Norfolk, VA, North Carolina State University and WebAssign, Raleigh, NC, North Carolina State University, Raleigh, NC, Broughton High School, Raleigh, NC, University of Wisconsin at Whitewater, Whitewater, WI, University of North Carolina-Chapel Hill Department of Physics, University of West Virginia - Chemistry Dept., ORNL, Old Dominion University, University of West Virginia Department of Chemistry, Clemson University, South Carolina Governor’s School for Science and Mathematics, College of Charleston, Rice University, Korea Research Institute of Standards and Science, Ohio State University, Wright State University, Yale U, JINR, Dubna, Univ. Fed. do Rio de Janeiro, Vanderbilt Univ./JIHIR(ORNL)/Tsinghua Univ., NSCL(MSU), LLNL, LBNL, Vanderbilt Univ./LBNL/JIHIR(ORNL), Vanderbilt Univ., Jagellonian University, University of Bonn, North Carolina A{\&T} State University, North Carolina Central University, Duke University and TUNL

  • J. Deng

    US Naval Observatory, University of Virginia, Thomas Jefferson National Accelerator Facility, College of William and Mary, US Army Research Office, Brimrose Corporation of America, Hampton University, Department of Physics, University of Rajshahi, Rajshahi, Bangladesh, Department of Physics, Old Dominion University, Norfolk, VA 23529, University of North Carolina at Chapel Hill, Department of Physics, Old Dominion University, Norfolk, Virginia, Department of Physics, Korea University, Seoul, Korea, Department of Physics, University of Virginia, Charlottesville, VA, Department of Physics, Old Dominion University, Norfolk, VA, North Carolina State University and WebAssign, Raleigh, NC, North Carolina State University, Raleigh, NC, Broughton High School, Raleigh, NC, University of Wisconsin at Whitewater, Whitewater, WI, University of North Carolina-Chapel Hill Department of Physics, University of West Virginia - Chemistry Dept., ORNL, Old Dominion University, University of West Virginia Department of Chemistry, Clemson University, South Carolina Governor’s School for Science and Mathematics, College of Charleston, Rice University, Korea Research Institute of Standards and Science, Ohio State University, Wright State University, Yale U, JINR, Dubna, Univ. Fed. do Rio de Janeiro, Vanderbilt Univ./JIHIR(ORNL)/Tsinghua Univ., NSCL(MSU), LLNL, LBNL, Vanderbilt Univ./LBNL/JIHIR(ORNL), Vanderbilt Univ., Jagellonian University, University of Bonn, North Carolina A{\&T} State University, North Carolina Central University, Duke University and TUNL

  • J. Deng

    US Naval Observatory, University of Virginia, Thomas Jefferson National Accelerator Facility, College of William and Mary, US Army Research Office, Brimrose Corporation of America, Hampton University, Department of Physics, University of Rajshahi, Rajshahi, Bangladesh, Department of Physics, Old Dominion University, Norfolk, VA 23529, University of North Carolina at Chapel Hill, Department of Physics, Old Dominion University, Norfolk, Virginia, Department of Physics, Korea University, Seoul, Korea, Department of Physics, University of Virginia, Charlottesville, VA, Department of Physics, Old Dominion University, Norfolk, VA, North Carolina State University and WebAssign, Raleigh, NC, North Carolina State University, Raleigh, NC, Broughton High School, Raleigh, NC, University of Wisconsin at Whitewater, Whitewater, WI, University of North Carolina-Chapel Hill Department of Physics, University of West Virginia - Chemistry Dept., ORNL, Old Dominion University, University of West Virginia Department of Chemistry, Clemson University, South Carolina Governor’s School for Science and Mathematics, College of Charleston, Rice University, Korea Research Institute of Standards and Science, Ohio State University, Wright State University, Yale U, JINR, Dubna, Univ. Fed. do Rio de Janeiro, Vanderbilt Univ./JIHIR(ORNL)/Tsinghua Univ., NSCL(MSU), LLNL, LBNL, Vanderbilt Univ./LBNL/JIHIR(ORNL), Vanderbilt Univ., Jagellonian University, University of Bonn, North Carolina A{\&T} State University, North Carolina Central University, Duke University and TUNL

  • J. Deng

    US Naval Observatory, University of Virginia, Thomas Jefferson National Accelerator Facility, College of William and Mary, US Army Research Office, Brimrose Corporation of America, Hampton University, Department of Physics, University of Rajshahi, Rajshahi, Bangladesh, Department of Physics, Old Dominion University, Norfolk, VA 23529, University of North Carolina at Chapel Hill, Department of Physics, Old Dominion University, Norfolk, Virginia, Department of Physics, Korea University, Seoul, Korea, Department of Physics, University of Virginia, Charlottesville, VA, Department of Physics, Old Dominion University, Norfolk, VA, North Carolina State University and WebAssign, Raleigh, NC, North Carolina State University, Raleigh, NC, Broughton High School, Raleigh, NC, University of Wisconsin at Whitewater, Whitewater, WI, University of North Carolina-Chapel Hill Department of Physics, University of West Virginia - Chemistry Dept., ORNL, Old Dominion University, University of West Virginia Department of Chemistry, Clemson University, South Carolina Governor’s School for Science and Mathematics, College of Charleston, Rice University, Korea Research Institute of Standards and Science, Ohio State University, Wright State University, Yale U, JINR, Dubna, Univ. Fed. do Rio de Janeiro, Vanderbilt Univ./JIHIR(ORNL)/Tsinghua Univ., NSCL(MSU), LLNL, LBNL, Vanderbilt Univ./LBNL/JIHIR(ORNL), Vanderbilt Univ., Jagellonian University, University of Bonn, North Carolina A{\&T} State University, North Carolina Central University, Duke University and TUNL