Direct Aminoglycoside Coated Gold Nanoparticles Synthesis, Characterization and Antibacterial Susceptibility Testing

POSTER

Abstract

Need for novel, innovative strategies for developing antibiotics is becoming a necessity due to an increasing number of rapidly evolving multi drug resistant bacteria threats. Antibiotic encapsulated gold nanoparticles (GNP's) are one such strategy showing promise. Kanamycin is an aminoglycoside antibiotic with bactericidal activity that works through binding the 30S subunit of the bacterial ribosome, interrupting protein synthesis. In this study we report development of Kanamycin coated gold nanoparticles (Kan-GNPs) which has highly effective, dose dependent antibacterial activity). The method employed in this study for synthesizing Kan-GNP's is an entirely eco-friendly, single step reaction, carried out within an aqueous buffer. The GNPs were identified and size was determined with transmission electron microscopy. The average diameter of the particles is 15 $\pm$ 5 nm. Further characterization was carried out with UV/vis spectrophotometer; Electron dispersion spectroscopy (EDS) and FTIR analysis. We have tested the antibacterial activity of Kan-GNP's against multiple strains of bacteria, both Gram-positive and Gram-negative, and have found Kan-GNP's to be highly efficient against all tested strains.

Authors

  • Rammohan Paripelly

    Western Kentucky University

  • Rajalingam Dakshinamurthy

    Western Kentucky University

  • J.K. Hwang

    Western Kentucky University, University of Pardubice, Francis Marion University, Clemson University Professor, Francis Marion University Professor, Undergraduate Administrator, Oak Ridge National Laboratory, Oak Ridge, Tennessee, Austin Peay State University, University Strenwarte-Muenchen, Seoul National University, Gatton Academy for Science and Mathematics, Alabama A\&M University, Cygnus, Center for Nanophase Materials Science at Oak Ridge National Laboratory, Vanderbilt University, Fisk Univ, 2Cornell High Energy Synchrotron Source, Cornell University, Ithaca, NY, NOVA Center, Western Kentucky University, Department of Physics, Florida A\&M University, Tallahassee, FL-32307, Correlated Electron Materials Group, Oak Ridge National Laboratory, Oak Ridge, Tennessee 37831-6061 USA, Department of Electrical Engineering and Computer Science, Vanderbilt University, Nashville, TN 37235, USA, Department of Electrical and Computer Engineering, University of Rochester, Rochester, NY 14627, USA, The Institute of Optics, University of Rochester, Rochester, NY 14627, USA, Universidade Estadual Paulista (UNESP), Clark Atlanta University, Deapartment of Physics \& Astronomy, Georgia State University, USA, Department of Electrical and Computer Engineering, McGill University, Montreal, QC H3A 2A7, Canada, Oak Ridge National Laboratory, University of South Alabama, Samford University, University of Rochester, University of North Carolina, Chapel Hill, Sandia National Laboratories, New Mexico State University, University of Tennessee Space Institute, Shanghai Jiao Tong University, Shanghai, China, University of Leeds, Leeds, UK, Georgia State University, Atlanta GA, University of Alabama at Birmingham, National High Magnetic Field Laboratory, Prairie View A\&M University, Brookhaven National Laboratory, University of Southern Indiana, Center for Nanophase Materials Sciences at Oak Ridge National Laboratory, JINR(Dubna), Tsinghua Univ., LBNL, Vanderbilt Univ., Vanderbilt Univ./Univ. of Tennessee, Knoxville, Vanderbilt Univ./Univ. of Kentucky, GANIL, Vanderbilt Univ./Union Univ., JINR, ORAU, Tsinghua University, LNBL