\textbf{Roco4 kinase crystal structures fail to capture expected dynamical behavior of LRRK2, a protein implicated in Parkinson's disease}

POSTER

Abstract

Leucine-rich repeat kinase 2 (LRRK2) is a multi-domain protein implicated in Parkinson's disease (PD). Understanding LRRK's contribution to pathogenesis requires study of the kinase domain. Unfortunately, such studies are complicated by the unavailability of LRRK2 crystal structures. Previous studies have shown that humanized Roco4 kinase may be a suitable surrogate for LRRK2 in chemical investigations. Our study assesses the dynamical behavior of humanized Roco4, compared to that of a homology model, by molecular dynamics simulations. We attempt to capture conformational changes of the activation loop from active to inactive forms, a key feature in the regulation of LRRK2 kinase activity. Our results indicate that simulations of humanized Roco4 fail to capture these conformational changes. Such a finding suggests that Roco4 may not be a suitable model for computational LRRK2 studies. We intend to conduct additional studies to further elucidate Roco4's performance as a LRRK2 mimic, though our initial results call into question its viability. A validated LRRK2 model is crucial for the development of drugs that can treat or even cure PD, and predictions of increasing disease frequency further compound the importance of LRRK2 research.

Authors

  • Sirui Ma

    Vanderbilt Univ

  • Richard Longland

    University of Tennessee, Knoxville, University of Alabama at Birmingham, Rutgers, The State University of New Jersey, Florida Intl Univ, Christopher Newport University, Jefferson Laboratory, University of North Georgia, Dahlonega GA 30597, Oak Ridge National Laboratory, Oak Ridge, TN 37831, Austin Peay State University department of physics and astronomy, Laboratoire des Verres et et Ceramiques, Institute des Sciences chimiques de Rennes, Austin Peay State University Department of Physics and Astronomy, Université de Rennes, Lehigh University, Austin Peay State University, University of Alabama, Oak Ridge National Laboratory, Auburn University, Auburn, AL, Purdue University, West Lafayette, IN, Queen's University Belfast, Belfast, United Kingdom, Harvard Smithsonian Center for Astrophysics, Boston, MA, Francis Marion University, Auburn University, Lawrence Berkeley National Laboratory, University of California, Davis, University of Alabama Huntsville, Oak Ridge National Lab, Univ of Tennessee, Knoxville, Oak Ridge National Lab, Univ of Tennessee, Knoxville, ITEP, Moscow, Baylor University, Saint Louis University, Louisiana State Univ - Baton Rouge, University of Chicago, Oak Ridge National Laboratory , Oak Ridge TN 37831, American Superconductor Corp., Westborough, MA 01581, USA, Tsinghua University, LBNL, Vanderbilt University, Laboratoire Aime Cotton, CNRS/Universite Paris-Sud/ENS-Cachan, Orsay France, Drake University, Des Moines, IA, University of Central Florida, University of South Alabama, North Carolina Central University