Heterogeneous distribution of kinesin–streptavidin complexes revealed by mass photometry

ORAL

Abstract

Kinesin–streptavidin complexes are widely used in microtubulebased active-matter studies. The stoichiometry of the complexes is empirically tuned but experimentally challenging to determine. Here, mass photometry measurements reveal heterogenous distributions of kinesin–streptavidin complexes. Our binding model indicates that heterogeneity arises from both the kinesin–streptavidin mixing ratio and the kinesin-biotinylation efficiency.

*This work was supported by the National Institutes of Health (R15 GM120682 to J. X.) and the Intramural Research Program of the National Heart, Lung, and Blood Institute, National Institutes of Health (ZIAHL001056 to K. C. N.).

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Presenters

  • Jing Xu

    • University of California, Merced

Authors

  • Jing Xu

    • University of California, Merced

  • Nathaniel Brown

    • University of California, Merced

  • Yeonee Seol

    • National Institutes of Health (NIH)

  • Keir C Neuman

    • National Institutes of Health (NIH)