Non-equilibrium regulation of condensate patterning

ORAL  · Invited

Abstract

Nucleolar fibrillar centers (FCs) form patterned condensates that are characterized by a typical condensate size, number and spacing in a cell. Experiments showed that inhibiting ribosomal RNA synthesis alters the patterning of FCs. Predictions from a field theory coupled to reaction-diffusion equations for RNA transcription predicted that active RNA synthesis generates a non-equilibrium state wherein condensate coarsening is arrested, thus resulting in patterned condensates. Experimental tests are in consistent with predictions. Functional importance of arrested coarsening and patterning will also be described.

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Publication: Active RNA synthesis patterns nuclear condensates, under revision at Cell Systems; https://www.biorxiv.org/content/10.1101/2024.10.12.614958v1

Presenters

  • Arup K Chakraborty

    • Massachusetts Institute of Technology

Authors

  • Arup K Chakraborty

    • Massachusetts Institute of Technology

  • Salman F Banani

    • Department of Pathology, The University of Chicago, Chicago, IL 60637, USA

  • Andriy Goychuk

    • Massachusetts Institute of Technology

  • Pradeep Natarajan

    • Massachusetts Institute of Technology

  • Ming M Zheng

    • Whitehead Institute for Biomedical Research, Cambridge, MA 02142

  • Haoran Wang

    • Department of Biological Sciences, Carnegie Mellon University, Pittsburgh, PA

  • Giuseppe Dall'Agnese

    • Whitehead Institute for Biomedical Research, Cambridge, MA

  • Jonathan E Henninger

    • Department of Biological Sciences, Carnegie Mellon University, Pittsburgh, PA

  • Mehran Kardar

    • Massachusetts Institute of Technology

  • Richard A Young

    • Whitehead Institute for Biomedical Research, Cambridge, MA