Precise Flow Regulation and Gradient Shaping in Microfluidic Devices via Bias-Controlled Mixers

This study investigates how Fallopian tube flows and other mechanical stressors affect the initiation and early progression of ovarian cancer, using devices that generate a suitable microscale flow spectrum. We examine microfluidic devices that produce two-dimensional concentration landscapes characterized by either linear or exponential gradients across a designated chamber. The flow rates are meticulously calibrated to 15, 45, 70, 100, and 150 μm/s, representing linear flows within the chamber. This range encompasses the flow rates observed in the Fallopian tubes as reported in scientific literature. We achieved a standard deviation of less than 1% in both flow control and concentration equilibrium during epifluorescence analyses. The concentration distribution is demonstrated across the gradient generator, the gradient chamber, and along the chamber. Our findings indicate that the mechanical parameters of these devices are appropriate for future investigations into ovarian cancer.