Dielectric Characterization of Moringa Seed-Derived Mo-CBP3 Protein

The chitin-binding protein Mo-CBP3, a seed-specific bioactive protein isolated from Moringa oleifera seeds, was analyzed using broadband dielectric spectroscopy (0.01 Hz–10 MHz, 273–293 K) at two solution concentrations. A distinct β-relaxation attributed to Mo-CBP3 molecular reorientation was observed, with dielectric strength scaling with concentration and relaxation time following Arrhenius behavior. Complementary studies on raw Moringa oleifera seeds and leaves—including proteomic profiling and X-ray diffraction (XRD) analyses—were performed to relate the dielectric behavior of seed-derived Mo-CBP3 to the native structural and compositional features of the seeds and leaves and provide an integrated view of protein dynamics and molecular organization.