Two-photon flow cytometer with non-diffracting beams

POSTER

Abstract

In vivo flow cytometry is used for medical diagnosis and the quantifying of circulating cells. This technique uses laser light for excitation to detect the fluorescent or acoustic signals after light is absorbed by molecules within the cell. A challenge in this approach is the limited detection depth due to the scattering of light by living tissue. Two-photon excitation based flow cytometers use infrared light and have longer detection depth, but limited sample volume. In this project a light-sheet microscope was made to increase the sample volume. In conventional flow cytometer Gaussian beams are commonly used, but it has limited sample volume as focused Gaussian beams diverge quickly after focus. So a self-healing and non-diffracting beam named Bessel beam is used to generate a light-sheet. Bessel beams have concentric rings that create background noise, but can be reduced through the use of two-photon excitation. This can be further improved by using Airy beams which have been shown to produce a three-fold increase in detection depth when compared to Bessel beams. In this study a scanning two-photon Airy beam light-sheet is implemented for greater resolution, sample size, and detection depth of microfluidic channels, therefore future in vivo flow cytometry.

Authors

  • Andres Reyes

    Univ of Texas, El Paso

  • Yu Ding

    Univ of Texas, El Paso

  • Qiye Zheng

    Santa Fe Institute, Baylor University, University of Texas at Dallas, Department of Chemistry, The University of Texas at Austin, Jozef Stefan Institute, Texas A&M University-Commerce, Commerce, Texas 75429, Cyclotron Institute, Texas A&M University, College Station, Texas 77843, Texas A&M University, Department of Physics, Texas State University, Department of Physics, Baylor University, University of Texas at El Paso, Univ of Texas, El Paso, University of Science and Technology of China, The University of Texas at Dallas, Faculty, None, Southwestern University, Texas State University, Texas A&M University - Commerce, UT Southwestern Medical Center, National High Magnetic Field Laboratory, The Cyclotron Institute at Texas A&M University, Department of Biological Sciences, Texas State Univ-San Marcos, The University of Texas at Dallas, Richardson, Texas 75080, King Abdullah University of Science and Technology, Univ of Texas, Dallas, N.Chiao Tung U., UT Dallas, Inorganic Chemistry and Catalysis Group, Utrecht University, Electrical & Computer Engineering, Baylor University, Department of Materials Science and NanoEngineering, Rice University, University of Texas at Arlington, University of Chicago, The University of Mississippi, Astronomical Observatory, Warsaw University, Nicolaus Copernicus Astronomical Centre, Polish Academy of Sciences, Rochester Institute of Technology, California Institute of Technology, University of Houston, NASA-GSFC and UMBC, MD, Virginia Tech, VA, Texas Christian University, The University of Texas at San Antonio, Department of Physics, Teivecca Nazarene University, Weatherford College, Air Force Research Laboratory, Sensors Directorate, WPAFB, OH, USA, Air Force Research Laboratory, Directed Energy Directorate, KAFB, NM, US, Department of Physics & Astronomy, University of Texas at San Antonio TX, USA, University of Arizona, University of North Carolina at Chapel Hill, Stanford University, Harvard Center for Astrophysics, Texas A\&M University, UTSW, University of Illinois at Urbana-Champaign, Urbana, Illinois 61801, Boston College, Chestnut Hill, Massachusetts 02467, Naval Research Laboratory, Washington, D.C. 20375

  • Chunqiang Li

    Univ of Texas, El Paso