Comparison of optical properties of cultured and excised ocular tissues as single- and multi-layered structures

ORAL

Abstract

The near infrared (NIR) optical properties of the various ocular layers, including the retinal pigmented epithelium (RPE), sclera, and choroid, were studied using a double-integrating sphere setup. Inverse Adding-Doubling, Kubelka-Munk, and Inverse Monte Carlo techniques were applied to obtain absorption and scattering coefficients. Changes in polarization of transmitted and reflected light were measured using a polarizer and single integrating sphere. ARPE24 cell monolayers were cultured in viewing windows and optically characterized. Newly excised ocular tissues were bisected and characterized as a single- and multi-layer tissue structures. The results from excised and cultured tissues were compared to determine any significant differences in the NIR optical properties. A comparison was also made between the NIR optical properties of the multi-layer retinal structure and a model of the multi-layer structure constructed from the optical properties of the single tissue-type layers. This study will add to the understanding of how light propagates through turbid media, such as biological tissues consisting of multiple cell-type layers.

–

Authors

  • Bruce Gnade

    Grand Valley State University, STC, Health Physics Society, The University of Texas at San Antonio, Texas Tech University, Lubbock, Texas, PIEAS, Pakistan, Texas Tech University, Lubbock, TX and PIEAS Pakistan, Rutgers University, Department of Physics, Texas A\&M University, College Station, TX 77843, Waxahachie Global High School, Waxahachie, TX, University of Texas at San Antonio, Tohoku University Institute of Materials Research, University of Alberta Department of Physics, Istanbul University Department of Physics, Geophysical Institute, Tohoku University, Japan, Planetary Plasma and Atmospheric Research Center, Tohoku University, Japan, Solar-Terrestrial Environment Laboratory, Nagoya University, Japan, Austin Community College, Dallas Baptist University, Angelo State University, Lake Highlands High School, Baylor University, Waco, TX, Texas Christian University, Fort Worth, TX, Paul Laurence Dunbar High School, Fort Worth, TX, Success High School, Fort Worth, TX, Dept. of Math., Univ. of New Mexico, Depts. of Materials Science and Engineering and Chemistry, University of Texas at Dallas

  • Brian Yust

    The University of Texas at San Antonio

  • Bruce Gnade

    Grand Valley State University, STC, Health Physics Society, The University of Texas at San Antonio, Texas Tech University, Lubbock, Texas, PIEAS, Pakistan, Texas Tech University, Lubbock, TX and PIEAS Pakistan, Rutgers University, Department of Physics, Texas A\&M University, College Station, TX 77843, Waxahachie Global High School, Waxahachie, TX, University of Texas at San Antonio, Tohoku University Institute of Materials Research, University of Alberta Department of Physics, Istanbul University Department of Physics, Geophysical Institute, Tohoku University, Japan, Planetary Plasma and Atmospheric Research Center, Tohoku University, Japan, Solar-Terrestrial Environment Laboratory, Nagoya University, Japan, Austin Community College, Dallas Baptist University, Angelo State University, Lake Highlands High School, Baylor University, Waco, TX, Texas Christian University, Fort Worth, TX, Paul Laurence Dunbar High School, Fort Worth, TX, Success High School, Fort Worth, TX, Dept. of Math., Univ. of New Mexico, Depts. of Materials Science and Engineering and Chemistry, University of Texas at Dallas

  • Bruce Gnade

    Grand Valley State University, STC, Health Physics Society, The University of Texas at San Antonio, Texas Tech University, Lubbock, Texas, PIEAS, Pakistan, Texas Tech University, Lubbock, TX and PIEAS Pakistan, Rutgers University, Department of Physics, Texas A\&M University, College Station, TX 77843, Waxahachie Global High School, Waxahachie, TX, University of Texas at San Antonio, Tohoku University Institute of Materials Research, University of Alberta Department of Physics, Istanbul University Department of Physics, Geophysical Institute, Tohoku University, Japan, Planetary Plasma and Atmospheric Research Center, Tohoku University, Japan, Solar-Terrestrial Environment Laboratory, Nagoya University, Japan, Austin Community College, Dallas Baptist University, Angelo State University, Lake Highlands High School, Baylor University, Waco, TX, Texas Christian University, Fort Worth, TX, Paul Laurence Dunbar High School, Fort Worth, TX, Success High School, Fort Worth, TX, Dept. of Math., Univ. of New Mexico, Depts. of Materials Science and Engineering and Chemistry, University of Texas at Dallas

  • Bruce Gnade

    Grand Valley State University, STC, Health Physics Society, The University of Texas at San Antonio, Texas Tech University, Lubbock, Texas, PIEAS, Pakistan, Texas Tech University, Lubbock, TX and PIEAS Pakistan, Rutgers University, Department of Physics, Texas A\&M University, College Station, TX 77843, Waxahachie Global High School, Waxahachie, TX, University of Texas at San Antonio, Tohoku University Institute of Materials Research, University of Alberta Department of Physics, Istanbul University Department of Physics, Geophysical Institute, Tohoku University, Japan, Planetary Plasma and Atmospheric Research Center, Tohoku University, Japan, Solar-Terrestrial Environment Laboratory, Nagoya University, Japan, Austin Community College, Dallas Baptist University, Angelo State University, Lake Highlands High School, Baylor University, Waco, TX, Texas Christian University, Fort Worth, TX, Paul Laurence Dunbar High School, Fort Worth, TX, Success High School, Fort Worth, TX, Dept. of Math., Univ. of New Mexico, Depts. of Materials Science and Engineering and Chemistry, University of Texas at Dallas