Nano-mechanics of prostate cancer cells on nano-scaffolds

POSTER

Abstract

We investigated the nano-mechanics of lowly metastatic (LNCaP) and highly metastatic (CL-1) prostate cancer cells cultured on nano-scaffolds by performing AFM indenting experiments. The functionalized nano-scaffolds allowed us to control the cell-to-substrate adhesion, hence the focal adhesion. We measured the elastic moduli at the center of the cell at different adhesion sizes of the nano-arrays. The obtained mechanical signature indicates that smaller focal adhesion could elicit apoptosis. This study, in general, demonstrates that nano-scaffolds could be used as a tool for adhesion assay and as a~metastatic indicator.

Authors

  • Lyndon Bastatas

    Texas Tech University

  • James Matthews

    Texas Tech University

  • Brandon Cavness

    Texas A\&M University, University of Punjab Lahore Pakistan, Department of Physics, Lamar University, Beaumont, TX, BAE Systems, Advanced Systems and Technology, Nashua, NH 03061-0868, USA, Istanbul University, Beyazit, 34459 Istanbul, Turkey, Texas Tech University, Lubbock, TX 79409-1051, USA, Texas Tech University, Department of Physics and Astronomy, Texas A\&M University, Los Alamos National Laboratory, Texas A\&M University-Commerce, ATLAS Collaboration, Physics Department,The University of Texas at Dallas, Department of Surgery (Emergency Medicine), The University of Texas Southwestern Medical Center at Dallas, Texas Tech University - Health Science Center, Massachusetts Institute of Technology, San Antonio College, Angelo State University, Angelo State University / Los Alamos National Laboratory

  • Brandon Cavness

    Texas A\&M University, University of Punjab Lahore Pakistan, Department of Physics, Lamar University, Beaumont, TX, BAE Systems, Advanced Systems and Technology, Nashua, NH 03061-0868, USA, Istanbul University, Beyazit, 34459 Istanbul, Turkey, Texas Tech University, Lubbock, TX 79409-1051, USA, Texas Tech University, Department of Physics and Astronomy, Texas A\&M University, Los Alamos National Laboratory, Texas A\&M University-Commerce, ATLAS Collaboration, Physics Department,The University of Texas at Dallas, Department of Surgery (Emergency Medicine), The University of Texas Southwestern Medical Center at Dallas, Texas Tech University - Health Science Center, Massachusetts Institute of Technology, San Antonio College, Angelo State University, Angelo State University / Los Alamos National Laboratory

  • Brandon Cavness

    Texas A\&M University, University of Punjab Lahore Pakistan, Department of Physics, Lamar University, Beaumont, TX, BAE Systems, Advanced Systems and Technology, Nashua, NH 03061-0868, USA, Istanbul University, Beyazit, 34459 Istanbul, Turkey, Texas Tech University, Lubbock, TX 79409-1051, USA, Texas Tech University, Department of Physics and Astronomy, Texas A\&M University, Los Alamos National Laboratory, Texas A\&M University-Commerce, ATLAS Collaboration, Physics Department,The University of Texas at Dallas, Department of Surgery (Emergency Medicine), The University of Texas Southwestern Medical Center at Dallas, Texas Tech University - Health Science Center, Massachusetts Institute of Technology, San Antonio College, Angelo State University, Angelo State University / Los Alamos National Laboratory

  • Brandon Cavness

    Texas A\&M University, University of Punjab Lahore Pakistan, Department of Physics, Lamar University, Beaumont, TX, BAE Systems, Advanced Systems and Technology, Nashua, NH 03061-0868, USA, Istanbul University, Beyazit, 34459 Istanbul, Turkey, Texas Tech University, Lubbock, TX 79409-1051, USA, Texas Tech University, Department of Physics and Astronomy, Texas A\&M University, Los Alamos National Laboratory, Texas A\&M University-Commerce, ATLAS Collaboration, Physics Department,The University of Texas at Dallas, Department of Surgery (Emergency Medicine), The University of Texas Southwestern Medical Center at Dallas, Texas Tech University - Health Science Center, Massachusetts Institute of Technology, San Antonio College, Angelo State University, Angelo State University / Los Alamos National Laboratory