Utilizing Functionalized Nano-Paterned Surfaces as a clue to Cell Metastasis in Prostate and Breast Cancer

ORAL

Abstract

There is a direct relation between the survival of a patient diagnosed with prostate or breast cancer and the metastatic potential of the patient's cancer. It is therefore extremely important to prognose metastatic potentials. In this study we investigated whether the behaviors of cancer cells responding to our state of the art nano-patterns differ by the metastatic potential of the cancer cells. We have used lowly (LNCaP) and highly (CL-1) metastatic human prostate cancer cells and lowly (MCF-7) and highly (MB231) metastatic breast cancer cells. A surface functionalization study was then performed first on uniform gold and glass surfaces, then on gold nano-patterned surfaces made by nano-sphere lithography using nano-spheres in diameter of 200nm to 800nm. The gold surfaces were functionalized with fibronectin (FN) and confirmed through XPS analysis. The CL-1, MCF-7, and MB231 cells show similar proliferation on all surfaces regardless of the presence of FN, whereas LNCaP show a clear preference for FN coated surfaces. The proliferation of the LNCaP was reduced when grown on finer nano-scaffolds, but the more aggressive CL-1, MB231, and MCF-7 cells show an abnormal proliferation regardless of pattern size. The difference in adhesion is intrinsic and was verified through dual fluorescent imaging. Clear co-localization of actin-vinculin were found on CL-1, MCF-7, and MB231. However LNCaP cells showed the co-localization only on the tips of the cells. These results provide vital clues to the bio-mechanical differences between the cancer cells with different metastatic potential.

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Authors

  • James Matthews

    Texas Tech University

  • Brandon Cavness

    Texas A\&M University, University of Punjab Lahore Pakistan, Department of Physics, Lamar University, Beaumont, TX, BAE Systems, Advanced Systems and Technology, Nashua, NH 03061-0868, USA, Istanbul University, Beyazit, 34459 Istanbul, Turkey, Texas Tech University, Lubbock, TX 79409-1051, USA, Texas Tech University, Department of Physics and Astronomy, Texas A\&M University, Los Alamos National Laboratory, Texas A\&M University-Commerce, ATLAS Collaboration, Physics Department,The University of Texas at Dallas, Department of Surgery (Emergency Medicine), The University of Texas Southwestern Medical Center at Dallas, Texas Tech University - Health Science Center, Massachusetts Institute of Technology, San Antonio College, Angelo State University, Angelo State University / Los Alamos National Laboratory