Combinatorial role of mechano-chemical cues in cell mechanics and state transitions

Cells typically reside in complex physiological milieus which exhibit spatiotemporally heterogeneous material properties and diverse signalling cues. Two such widely studied microenvironmental parameters are mechanical stimuli and chemical sensing, which are themselves modulated by cellular activity. For instance, complex physiological processes such as embryogenesis and tissue patterning feature dynamic ECM remodelling and chemotactic migration by cells. However, conventional approaches largely study these players in isolation, despite substantial experimental evidence hinting at extensive crosstalk and cross-regulation. Our present work will explore a combinatorial mechano-chemical phase space that influences the mechanical state of cells and regulates cell state transitions. Leveraging quantitative measurements and phenotypic assays at both the collective and single cell levels, we will characterise this conjoined axis.